DNA Extraction and Purification
Research Products International Corp Zymoprep Yeast Plasmid Miniprep II w/Zymo-Spin I Columns, Uncapped, 50 Preps
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
Simple: Quickly and easily rescue plasmid from yeast.
Research Products International Corp Genomic DNA Clean & Concentrator-10 Kit, 25 Preps
The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
New England Biolabs, Inc. Monarch® HMW gDNA Tissue Lysis Buffer - 62 ml
The Monarch HMW gDNA Tissue Lysis Buffer, supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue, is a highly effective lysis buffer for tissue and bacteria samples. Its composition ensures immediate and complete inactivation of nucleases in the samples at the start of the lysis.
- Supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue
- This lysis buffer enables rapid and effective lysis of various tissue and bacteria samples
- Offers maximal protection against nucleases allowing for the isolation of intact HMW DNA
- Supplied as one bottle of 62 ml
Macherey-Nagel NucleoSpin Soil, 50 preps
NucleoSpin Soil (50) 50 preps for the isolation of DNA from soil and sediment - NucleoSpin Soil Columns, NucleoSpin Bead Tubes, NucleoSpin Inhibitor Removal Columns, Collection Tubes, buffers
Zymo Research Corporation ZymoBIOMICS BashingBead Buffer, 150 ml
ZymoBIOMICS BashingBead Buffer 150 mL
Zymo Research Corporation Zyppy™ Elution Buffer (5 ml)
Zyppy Elution Buffer is designed to be used with our Zyppy Plasmid Miniprep Kit, which uses a pellet-free modified alkaline lysis method to isolate ultra-pure plasmid DNA from E. coli in only 8 minutes.
Zymo Research Corporation EZ-96 DNA Methylation MagPrep (8x96 rxns.)
The EZ-96 DNA Methylation MagPrep features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Quick-DNA Plant/Seed 96 Kit (2 x 96 Preps)
The procedure is easy and can be completed in as little as 50 minutes: plant samples ( 80 mg each) are added directly to the tubes of a ZR BashingBead™ Lysis Rack (2.0 mm) and are rapidly and efficiently lysed by bead beating (e.g., 2010 GenoGrinder Instrument, page 5) without the use of organic denaturants or proteinases. Polysaccharides and polyphenols/tannins are removed from the DNA using our Zymo-Spin™ 96-well plate and Silicon-A™-HRC Plate technologies, respectively. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Zymo Research Corporation MagBead DNA/RNA Wash 2, 20 ml
MagBead DNA/RNA Wash 2 for use with several Zymo Research Magnetic bead based kits for high-throughput automation of DNA and RNA purification.
ATCC Quantitative Genomic DNA; from Babesia microti; Strain: GI (Ingram strain)
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load.
