DNA Extraction and Purification
Macherey-Nagel Resuspension Buffer RES‑EF, 1000 mL
Resuspension Buffer RES‑EF to be used with NucleoBond Xtra Midi / Maxi EF kits.
Bioventures Inc DNA/RNA ISOLATION
MAPMARKER, 50-1000BP, TMR Labeled (50, 75, 100, 125, 150, 200, 250, 300, 350, 400, 450, 475, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000bp), 8fmoles/band/ul Concentration
Zymo Research Corporation ZymoBIOMICS 96 MagBead DNA/RNA Kit (96 Preps.)
The provided DNA/RNA Shield™ inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into 50 l of ZymoBIOMICS™ DNase/RNaseFree Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
New England Biolabs, Inc. Monarch® gDNA Elution Buffer - 34 ml
Monarch gDNA Elution Buffer is supplied in 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA and is suitable for long term storage of gDNA. Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution. This buffer is a component of the Monarch Genomic DNA Purification Kit.
- A component of the Monarch Genomic DNA Purification Kit
- 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA
- Suitable for long term storage of gDNA
- Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution
- Monarch kit components are also available separately for added convenience
New England Biolabs, Inc. Monarch® Spin gDNA Extraction Kit - 150 preps
The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms
New England Biolabs, Inc. Monarch® gDNA Purification Columns - 100 columns
The Monarch gDNA Purification Columns are a component of the Monarch Genomic DNA Purification Kit and can be used to purify up to 30 ug genomic DNA from a variety of sample types. They are available for purchase separately for your convenience and supplied with Monarch Collection Tubes II.
- A component of the Monarch Genomic DNA Purification Kit
- Can be used to purify up to 30 ug genomic DNA from a variety of sample types
- Contains 100 columns and 100 collection tubes
- Compatible with Monarch Collection Tubes II
- Monarch kit components are also available separately for added convenience
New England Biolabs, Inc. Exo-CIP Rapid PCR Cleanup Kit – 400 reactions
The Exo-CIP Rapid PCR Cleanup Kit contains optimized formulations of thermolabile Exonuclease I and thermolabile Calf Intestinal Phosphatase (CIP) and is used to rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification. Degradation occurs in only 4 minutes at 37C, and is immediately followed by rapid inactivation of the enzymes by heating for 1 minute at 80C. In just 5 minutes, the PCR product is ready for downstream analysis, such as Sanger Sequencing, SNP detection, or library preparation for NGS. The Exo-CIP Rapid PCR Cleanup Kit is compatible with all commonly-used PCR buffers.
Boca Scientific Inc MagSi-DNA Trial Kit, Magnetic Beads for DNA/RNA Purification, 8 Types of Beads, 2ml each
The MagSi-DNA Trial Kit includes 2ml of each of the 8 types of MagSi-DNA magnetic beads for nucleic acid isolation and purification. The beads in this trial kit include both silica and carboxylated surfaces, pore sizes of 300nm, 600nm, 1.2um and 3um, and ferromagnetic options. With the variety of different properties of the beads in this kit, the user can determine which beads are ideal for the application. MagSi-DNA beads are intended as a solid phase extraction tool for custom buffer systems based on chaotropic and non-chaotropic binding principles. They have a high binding capacity for nucleic acids and are well-suited for developing your own nucleic acid isolation methods. As no columns or centrifugation steps are required, MagSi-DNA beads are ideal for high-throughput and automated applications.
Norgen Biotek Corp Oligo Clean-Up/Concentrat Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling. Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction. The kit provides a high quality product with up to 90% recovery. Purified oligos can be used in a number of downstream applications such as end-point or quantitative reverse transcription PCR and RNAi studies.
Agilent Technologies HS Large Fragment 50KB Kit, 500 samples, DNA Sizing Range: 75 bp - 50000 bp, DNA Sizing Accuracy: ± 15%, DNA Concentration Range: 5 pg/μL - 600 pg/μL, DNA Smear Range: 50 pg/μL - 5 ng/μL, Sample Volume Required: 2 μL
HS Large Fragment 50KB Kit, 500 samples, DNA Sizing Range: 75 bp - 50000 bp, DNA Sizing Accuracy: ± 15%, DNA Concentration Range: 5 pg/μL - 600 pg/μL, DNA Smear Range: 50 pg/μL - 5 ng/μL, Sample Volume Required: 2 μL
Research Products International Corp DNA Clean & Concentrator-25 w/Zymo-Spin II Columns, Uncapped, 200 Preps
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.